هذه الكتب هي مستقبل الطب
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A comprehensive and up-to-date set of basic and advanced methods that illuminates the principles of molecular diagnostics and allows researchers to establish assay systems tailored to their specific needs. This unique collection has been thoroughly updated and expanded and covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens and their use as diagnostic reagents in immunological assays. This new edition places significant emphasis on bioinformatics and proteomics and the characterization and modification of proteins. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Rather than focusing on particular infectious agents, this book focuses on the techniques that can be used and tailored to accomplish any kind of molecular diagnostic application in the clinical laboratory, thus making it an essential book for all laboratory disagnosticians.
Contents:
1. Identification of Novel Diagnostic Markers by Differential Display
Peng Liang; Fellan Wang; Welmin Zhu; Robert O'Connell; Lidia Averboukh
pp. 3-13
2. lmmunoprecipitation
Kari Johansen; Lennart Svensson
pp. 15-28
3. RTPCR Methods and Applications
Paul Siebert
pp. 29-54
4. Quantitative RT-PCR
Paul Siebert
pp. 55-80
5. Cloning cDNA Ends Using RACE
Yue Zhang; Michael Frohman
pp. 81-106
6. A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains
Robert Mierendorf; Beth Hammer; Robert Novy
pp. 107-124
7. Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis
Ulla Wienhues; Wolfgang Kraas; Christoph Seidel; Hans Ihlenfeldt; Günther Jung
pp. 125-142
8. Amplification of Genomic DNA by PCR
Bruno Frey
pp. 143-156
9. PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli
Udo Reischl
pp. 157-168
10. Application of TEV Protease in Protein Production
Deborah Polayes; T. Parks; Stephen Johnston; William Dougherty
pp. 169-184
http://rapidshare.com/files/292570/0...s_Diseases.pdf
كود:Table of Contents I. Recombinant Proteins and Peptides. Identification of Novel Diagnostic Markers by Differential Display Peng Liang, Feilan Wang, Weimin Zhu, Robert P. O'Connell, and Lidia Averboukh Immunoprecipitation Kari Johansen and Lennart Svensson RT-PCR Methods and Applications Paul D. Siebert Quantitative RT-PCR Paul D. Siebert Cloning cDNA Ends Using RACE Yue Zhang and Michael A. Frohman A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains Robert C. Mierendorf, Beth Hammer, and Robert E. Novy Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Finetuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis Ulla Wienhues, Wolfgang Kraas, Christoph Seidel, Hans Georg Ihlenfeldt, and Günther Jung Amplification of Genomic DNA by PCR Bruno Frey PCR-Based Cloning and Subsequent Expression of Antigenic Proteins in Escherichia coli Udo Reischl Application of TEV Protease in Protein Production Deborah A. Polayes, T. Dawn Parks, Stephen A. Johnston, and William G. Dougherty Stable Transfected Drosophila Schneider-2 Cells as a Novel Tool to Produce Recombinant Antigens for Diagnostic, Therapeutic, and Preventive Purposes Ludwig Deml and Ralf Wagner Use of Recombinant Antigens as Diagnostic Reagents in Enzyme-Linked Immunosorbent Assays Alan Warnes, Anthony R. Fooks, and John R. Stephenson Generation of Recombinant Baculovirus DNA in E. coli Using a Baculovirus Shuttle Vector Valentina C. Ciccarone, Deborah A. Polayes, and Verne A. Luckow The SFV Gene Expression System Valentina C. Ciccarone, Joel A. Jessee, and Peter Liljeström Expression and Purification of Recombinant Proteins Using the pET System Robert C. Mierendorf, Barbara B. Morris, Beth Hammer, and Robert E. Novy Procedures for the Analysis and Purification of His-Tagged Proteins Richard E. Kneusel, Joanne Crowe, Melanie Wulbeck, and Joachim Ribbe Detection and Immobilization of Proteins Containing the 6xHis Tag Richard E. Kneusel, Melanie Wulbeck, and Joachim Ribbe The Use of Recombinant Fusion Proteases in the Affinity Purification of Recombinant Proteins Louis E. C. Leong Purification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies) Udo Reischl Purification of Recombinant Proteins Having High Isoelectric Points Raffaele A. Calogero and Anna Aulicino Immunoblotting Michael Kirschfink and Peter Terness Diagnostic Application of a Multiple-Channel Immunoblot Apparatus Shaun D. Black Immobilization of Immunoreactants on Solid Phases for ELISAs Richard Decker and David Leahy Nonradioactive Labeling and Immunodetection of Recombinant Proteins Robert Oberfelder Flow Cytometry Volker Daniel Basic Problems of Serological Laboratory Diagnosis Walter Fierz Molecular Diagnostics Resources on the Internet Kevin J. O'Donnell II. Antibodies. Monoclonal Antibody-Based Immunoassays Peter Appleby and Udo Reischl Biosensor-Based Methods in Clinical Diagnosis Franz Aberl and Conrad Kößlinger The Quartz Crystal Microbalance (QCM) as an Immunosensor Franz Aberl, Conrad Kößlinger, and Hans Wolf Biomolecular Interaction Analysis Strategies for Antibody and Antigen Characterization Uwe W. Roder and Francis Markey Recent Developments in Antibody Engineering Melvyn Little, Stefan Dübel, Sergey Kipriyanov, and Frank Breitling Cloning and Expression of Single Chain Fragments (scFv) from Mouse and Rat Hybridomas Frank Breitling and Stefan Dübel Production of a Human Antibody Library in the Phage-Display Vector pSEX81 Martin Welschof, Melvyn Little, and Heinz Dörsam Screening of Phage-Displayed Antibody Libraries Heinz Dörsam, Michael Braunagel, Christian Kleist, Daniel Moynet, and Martin Welschof Purification, Characterization, and Biotinylation of Single-Chain Antibodies Sergey M. Kipriyanov Index.
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http://rapidshare.com/files/270901/0...s_Diseases.pdf
التعديل الأخير تم بواسطة Trojan ; 10-05-2007 الساعة 02:24 AM

هذه الكتب هي مستقبل الطب
جزاك الله خيرا




مشكوريييييييييييييييييييييييييييين وجزاك الله خير
THANKS![]()
ارجو منك اخي الكريم تحميل الكتاب مرة اخري لانه تم الغاؤه وشكرا لك
Diagnostic Moléculaire de Maladies Infectieuses
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Un jeu complet(compréhensif) et à jour des méthodes de **** et avancées qui illuminent les principes de diagnostic moléculaire et permet aux chercheurs d'établir des systèmes d'essai façonnés à leurs besoins spécifiques. Cette collection(ramassage) unique a été à fond mise à jour et étendue et couvre les dernières stratégies pour l'identification de nouveaux marqueurs diagnostiques, epitope la configuration et la production et la purification de recombinant antigens et leur utilisation comme des réactifs diagnostiques dans des essais immunologiques. Cette nouvelle édition place l'accent significatif sur bioinformatics et proteomics et la caractérisation et la modification de protéines. Il inclut aussi des techniques prometteuses basées sur des événements récents dans l'ingénierie d'anticorps et sur l'utilisation de biosensors. Plutôt que des agents se concentrant particuliers infectieux, ces centres de livre sur les techniques qui peuvent être employées et façonnés pour accomplir n'importe quelle sorte de demande(application) moléculaire diagnostique dans le laboratoire d'analyses médicales, le faisant ainsi un livre essentiel pour tout le laboratoire disagnosticians.
Contenu :
1. Identification de Nouveaux Marqueurs Diagnostiques par Exposition Différentielle
Peng Liang; Fellan Wang; Welmin Zhu; Robert O'Connell; Lidia Averboukh
Pp. 3-13
2. Lmmunoprecipitation
Kari Johansen; Lennart Svensson
Pp. 15-28
3. RTPCR Méthodes et Demandes(applications)
Paul Siebert
Pp. 29-54
4. RT-PCR quantitatif
Paul Siebert
Pp. 55-80
5. Clonage(multiplication) cDNA Fins Employant COURSE(RACE)
Yue Zhang; Michel Frohman
Pp. 81-106
6. Une Méthode pour l'Identification Rapide d'Epitopes et d'Autres Domaines de Peptide Fonctionnels
Robert Mierendorf; Beth Hammer; Robert Novy
Pp. 107-124
7. Configuration de B-cellule Linéaire Epitopes sur Polypeptides Viraux par Synthèse de Peptide Multiple et Sensibilité de Réglage d'accord Excellente et Spécificité du Peptide Identifié Antigens pour Demande(application) dans Diagnostic Viral
Ulla Wienhues; Wolfgang Kraas; Christoph Seidel; Hans Ihlenfeldt; Günther Jung
Pp. 125-142
8. Amplification d'ADN Génomique par PCR
Bruno Frey
Pp. 143-156
9. Clonage(multiplication) PCR-BASÉ et Expression Suivante de Protéines Antigenic dans Escherichia coli
Udo Reischl
Pp. 157-168
10. Demande(application) de TEV Protease dans Production de Protéine
Deborah Polayes; T. Parcs; Stéphane Johnston; William Dougherty
Pp. 169-184
بسم الله الرحمن الرحيم شكراً لك على هذه المشاركة وعليكم السلام ورحمة الله وبركاته
thank you tooo much
تم تجديد الرابط
شكرآ لكم
thank you for your effort
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